Journal: Molecular Metabolism

Article Title: Loss of Tbk1 kinase activity protects mice from diet-induced metabolic dysfunction

doi: 10.1016/j.molmet.2018.06.007

Figure Lengend Snippet: Tbk1 Δ/ Δ mice have less inflammation relative to Tbk1 +/ + mice on HFD . (A) Representative 20x images and quantification of cd11b/c stained subcutaneous white adipose tissue from Tbk1 Δ/Δ and Tbk1 +/+ mice after 10 weeks on ND or HFD (n = 4–8 mice/group). Scale bar indicates 200 μM and ‘CLS’ refers to crown like structures. (B) mRNA expression of genes encoding CD11c, F4/80, TNFα, IL-6 and IL-12 in subcutaneous WAT of Tbk1 Δ/Δ and Tbk1 +/+ mice fed with HFD as indicated (n = 4–6 mice/group). (C) Liver tissue lysates from 14-week-old HFD-fed Tbk1 Δ/Δ and Tbk1 +/+ mice were immunoblotted with antibodies against IL-6 and IL-1β. β-actin and GAPDH were used as internal loading controls. Indicated cytokines from WAT (D) and liver tissue (E) lysates of HFD-fed Tbk1 +/+ and Tbk1 Δ/Δ mice were measured by Bio-Rad multiplex array (n = 4–8 mice/group for WAT, n = 8–12 mice/group for liver). All mice are from 129S5 background. Results are representative of mean +/− SEM. Statistical analysis by Student's t -test. * p < 0.05, ** p < 0.01.

Article Snippet: Sections for immunohistochemical analysis were blocked with 20% aquablock and incubated with rabbit anti-cd11b/c (Novus Biologicals, NB110-40766) in blocking solution (5% BSA in TBS with 0.05% tween) at 4 °C overnight.

Techniques: Staining, Expressing, Multiplex Assay

Journal: Journal of neuroinflammation

Article Title: Microglia either promote or restrain TRAIL-mediated excitotoxicity caused by Aβ 1-42 oligomers.

doi: 10.1186/s12974-024-03208-2

Figure Lengend Snippet: Fig. 6 Microglial repopulation strongly reduces Aβ-induced neurotoxicity. (A) Experimental design for microglial repopulation in brain slice culture. (B) Expression of microglial genes at baseline, after depletion and after repopulation. Each data point represents the average of two technical replicates from different culture wells. 2-way ANOVA. F3,34=7.4, p = 0.0006. Tukey’s multiple comparisons test. ****p < 0.0001 Control vs. Depletion, ####p < 0.0001, Deple tion vs. Repopulation. N = 3–6/group. (C) Depiction of microglial isolation by CD11b + MACS. (D) Expression of microglial genes after MACS isolation in the microglial fraction and the remaining cells. 2-way ANOVA, F2,24=136.9, p < 0.00001****p < 0.0001 Sidak’s post-test. (E) Microglia were isolated from HEBSCs containing either native or RM by MACS. RM showed increased expression of BDNF compared to native microglia. (F) Represented image of PI staining in HEBSCs with either naïve or RM treated with Aβ1−42 (1µM) for 4 days. (G) Quantification of robust increases in neurotoxicity after Aβ1−42 treatment in HEBSCs with naïve, but not repopulated microglia. 1-way ANOVA, F2,37=21.72, p < 0.0001. N = 10–20 slices/group

Article Snippet: Samples were incubated with FcR blocking reagent (10 μL; Miltenyi Biotec, Cat. #130-092-575) for 10 min at 4 °C followed by incubation with 10 μL CD11b microbeads (Miltenyi Biotec, Cat. #130-105-634) at 4 °C for 15 min.

Techniques: Slice Preparation, Expressing, Control, Isolation, Staining

Journal: BMC Molecular and Cell Biology

Article Title: Transcriptome and proteome profiling reveal complementary scavenger and immune features of rat liver sinusoidal endothelial cells and liver macrophages

doi: 10.1186/s12860-020-00331-9

Figure Lengend Snippet: Antibodies used in the study

Article Snippet: CD11b/c Biotin (OX-42) , CD11b/c, CR3 , Cedarlane , CL042B , 2 μg/ml.

Techniques: Concentration Assay, Flow Cytometry, Control, Staining, FACS